Opportunities in Clinical Microbiology to Interface with Other Laboratory Disciplines

Attribute Sciences, Process Development, Amgen Biopharmaceuticals are drugs manufactured by growing genetically engineered cells in bioreactors to produce a therapeutic protein. Plastic single-use bioreactors are of interest to biopharmaceutical drug manufacturers due to their significant environmental and cost benefits and flexibility over stainless steel bioreactors. Effect of plastics on the biomanufacturing process is not yet completely understood. A case study on extractables from single-use bioreactors/bags and the impact on cell culture performance will be presented.

MicroRNA(miRNA) are short non-coding single-stranded RNA molecules that regulate gene expression at the post-transcriptional level. They are known to play a critical role in multiple biological processes including proliferation, differentiation, apoptosis, and hematopoiesis. Dysregulation of miRNA expression, therefore, is associated with a number of pathological conditions such as inflammation, cardiovascular diseases, neurological disorders and several types of cancers. miRNAs are also found to be extremely stable in body fluids such as serum, plasma, urine, breast milk, and saliva. Extracellular miRNAs are either packed into exosomes or loaded into high-density lipoproteins or bound by AGO2 proteins outside on vesicles. These modes of action protect miRNAs from degradation and improve their stability. Because of their stability in body fluids, miRNAs are gaining importance as biomarkers in liquid biopsies.

Join this 60-minute KnipBio webinar, to learn examples of engineering solutions based on biotechnology that will help the sustainable expansion of this field. Aquaculture, or the farming of fish, shrimp and seaweeds, is expected to continue its dramatic growth in the coming decades. One of the central issues for the continued growth of aquaculture will be the supply of high quality ingredients.

While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This has resulted in the development of scattering microscopy techniques, such as darkfield, iSCAT, COBRI, and Mass Photometry.