| | | HOST CELL PROTEIN CHARACTERISATION AND MASS SPECTROMETRY STRATEGIES | | Host cell proteins (HCPs) are process-related contaminants that are released to the cell culture supernatant due to secretion or lysis of host cells during the production of biologics. Due to the potential adverse effects of HCPs on the biotherapeutics stability, quality and efficacy, moni... | | |
| | | OFF-TARGET EFFECTS IN CRISPR-CAS9 GENOME EDITING: SECURING SPECIFICITY | | A cause for concern regarding the popular CRISPR-Cas9 technology is the potential occurrence of off-target effects. RNA-guided Cas9 may cleave DNA sequences that are not exact complements of the guide strand when either strand harbors bulge due to insertions or deletions. Undoubtedly, a be... | | |
| | | PDA TECHNICAL REPORT ON LOW ENDOTOXIN RECOVERY | | Since first reported by Chen and Vinther in 2013, the phenomenon known as low endotoxin recovery (LER) has been broadly observed in certain matrices commonly used for biologic formulations and certain therapeutic proteins. LER is defined as the inability to recover >50% activity over ti... | | |
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| | | IMAGING THE BRAIN AT HIGH SPATIOTEMPORAL RESOLUTION | | Physics has long employed optical methods to probe and manipulate matter on scales from the infinitesimal to the immense. To understand the brain, we need to monitor the physiological processes of single synapses as well as the neural activity of a large number of networked neurons. Optica... | | |
| | | STREAMLINED FORMULATION OF ENZYME PRODUCTS | | There are two complementary paths to creating a stable enzyme formulation. First, the enzyme itself may be engineered to increase its stability under storage conditions. However, this is not always possible, practical, or even preferable. Second, the enzyme can be formulated to maximize it... | | |