Pluripotent stem cells: How to raise the quality of your PSC culture

The past decade has witnessed an emergence of technologies from various disciplines that are providing unprecedented opportunities to formulate and answer new types of biological questions. In particular, single-molecule approaches are revolutionizing biological inquiries by providing previously unattainable data on elementary biological processes. Because biological molecules do not function in isolation in the cell, measurements need to be improved to better emulate in vivo conditions by studying more-complex systems with many components, and they need to be applied to the cellular levels and even tissue levels. This Cell Press webinar will discuss the latest advances in single molecule technologies, and the challenges and opportunities associated with their extension to very large scales and in interpreting the large and complex datasets.

A Virtual Special Issue of FEMS Yeast Research accompanies the webinar, gathering 19 recent research papers that illustrate the breadth of both fundamental and biotechnology-orientated research going on in the community.

This presentation will summarize the state-of-the-art in transporters in drug development based on four recent ITC3 whitepapers (Clin Pharmacol Ther 104, October 2018). Practical overview of emerging transporters of clinical relevance will be provided (e.g., OCT1, OATP2B1, etc.). Best practice in design of clinical DDI studies will be illustrated with recent advances in the study of OCT1, P-gp, and BCRP. Considerations for optimal selection of clinical probe drugs, including future potential utility of transporter biomarkers, also will be discussed.

In developing and manufacturing mRNA vaccines and therapeutics, selecting and using the appropriate quality or grade of mRNA raw materials can help you meet critical process, scale, quality, and regulatory needs.