Protein Therapeutics: Understanding the Biophysical Characterization Toolbox

Over the past 12 years, the Biotherapeutics Analytical Summit has established a forum for analytical scientists to explore new tools, novel approaches and best practices to speed innovation. Living in a pandemic era, scientists are even more determined to share their research and find opportunities to collaborate and advance drug development.

Everyone makes the best extracts. But are they optimized? And what does “the best” mean; does it mean high yield, high quality, or both? And what is quality anyway? Quality begins in the cultivation process and continues throughout the handling of the product from seed to sale. At OutCo, we’ve studied our production pipeline and extraction methodologies to offer optimized products to our patients. Finding the ideal balance between yield and consumer rating, we were able to optimize extraction protocols for controlled mixtures of compounds. Our custom methods enable us to create products with designer cannabinoid and terpene ratios, customized to patients’ and doctors’ needs. All of this was achieved in an efficient manner through the use of design of experiment (DoE) techniques, and continuous improvement of all processes.

siRNA, which stands for small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules. It is sometimes known as short interfering RNA or silencing RNA. Before diving into what exactly siRNA is, not to be confused with miRNA, it's important to know the function of RNAs.

Current liquid chromatography-mass spectrometry (LC-MS) methods used to detect monoclonal antibodies (mAbs) in biological samples are focused on targeted tryptic peptide quantitation, which requires that the mAbs undergo tryptic digestion before analysis. The use of digestion complicates the workflow and can affect the results obtained. Therefore, there is an increasing interest to simplify the workflow, by developing improved methods which can detect the intact antibody or larger antibody fragments.