Integration of On-line HPLC as a PAT Tool for CQA Monitoring and Control in Bioprocessing

Genome-scale CRISPR screens in cancer cell lines have enabled the unprecedented identification of genome-scale vulnerabilities and co-dependencies. However, a significant obstacle in identifying co-dependencies is that the range of genomic features defines the discovery space. Therefore, it is clear that paired perturbations are needed to comprehensively map co-dependencies and establish functional genetic interaction maps.

The COVID-19 pandemic has shown the world that the effectiveness of mRNA vaccine technology is undeniable. The potential for these vaccines to efficiently treat a wide range of communicable diseases is enormous, and the technology is poised to explode into the marketplace. However, effective mRNA vaccines are only as good as the materials and methodologies used to create them, as well as the analytical steps used to test their potency, stability, and clinical efficacy. Traditional analytical methods are slow and end up wasting investigators’ time and money. Advanced imaging and analysis platforms may hold the key to simplifying vaccine development and manufacturing bottlenecks, as they are fast and can be multiplexed to answer multiple scientific questions per analysis.

This technical video provides an overview of the methodologies, tools, and reagents used to generate multicellular tumor spheroids. Specifically, this video discusses the benefits of Cultrex® extracellular matrices for spheroid formation and analysis. Technical tips are provided for using colorimetric and fluorometric assays to evaluate cell proliferation and invasion of spheroids. This protocol video was originally recorded by Dr. Gabriel Benton at Trevigen, Inc. The Trevigen portfolio, including tools for creating 3-D and spheroid cultures, is now a part of R&D Systems, a Bio-Techne brand.

Successful development of advanced biotherapeutics relies on extensive characterization and tight quality control of protein interactions. Characterizing the binding properties of complex biomolecules, such as bispecific antibodies or reengineered probiotics, is a critical step.