More Efficient Bispecific Antibody Purification With Fewer Steps

February 28, 2019

A single-step downstream process using protein A chromatography to purify a bispecific antibody (BsAb) in early screening has been successfully developed to replace an existing-three step process. The goal was to separate BsAb monomer from fragments such as the individual heavy and light chain pairs. MabSelect PrismA protein A resin was selected for the capture step. By applying a pH gradient elution from pH 5 to 3.5, most of the fragments could be removed as early as the capture step and without the need for additional purification steps. Target BsAb was efficiently purified with high recovery and purity using the single-step process described.

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Cynvenio Biosystems, Inc.

Cynvenio Biosystems, Inc. is a leader in the development of more affordable and clinically actionable precision medicine strategies for the individual cancer patient.

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