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Monitoring receptor ligand binding in living cells

December 17, 2018

A number of assay techniques are used to study ligand binding to GPCRs in living cells. The most well-known being the use of radioactively labelled ligands which unfortunately, is labor intensive, needs dedicated equipment and has disposal and safety issues associated with its use. More recently FRET (Fluorescence Resonance Energy Transfer), and Time Resolved-FRET techniques have also been used. However, fluorescence techniques require excitation with a high energy lamp and as cells display substantial auto-fluorescence this often leads to high background signal in the data. TR-FRET addresses this issue, but requires chemically based lanthanide labelling of receptors.

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