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Is A Rapid Cooling Step Needed When Freezing Cells For Cryopreservation?

March 14, 2018

Successful cell cryopreservation requires a controlled cooling rate. Cooling too rapidly or too slowly will lead to a poorer outcome. This is true because of the physical environment cells experience during cryopreservation. As ice forms in the extracellular solution, pure water is locked away like ice. As a result, cells are suspended in an increasingly concentrated solution, which dehydrates them. Cooling too slowly makes this solution toxic; cooling too rapidly prevents cells from dehydrating sufficiently, and intracellular ice forms.

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