How and when to use multiplexed immunofluorescence imaging in your research

Multiplexed tissue analysis is a powerful technique that allows comparisons of cell-type locations and cell-type interactions within a single fixed tissue sample. It is common for researchers to ask these questions before a multiplexing study begins: "How do I know what biomarkers are relevant in the tissue? Also, how do I pivot towards additional biomarkers as my research questions evolve?" Smart study design can help answer existing questions and enable continued exploration of new connections that weren’t immediately obvious at the beginning of the study. Multiplexed IF imaging can replace historical immunohistochemical staining, allowing researchers to explore more in-depth questions on the same piece of tissue.