Epidemiology, Clinical Features, and Diagnostic Testing

DNA assembly is commonly performed in the build step of the design-build-test-learn cycle at the core of synthetic biology. In many assembly workflows, DNA is assembled into gene fragments, which are then assembled into larger constructs, often plasmids.

Human induced pluripotent stem cells (hiPSCs) have been globally recognized as a multipurpose research tool for modeling human disease and biology, screening and developing potential therapeutic drugs, and implementing cell and gene therapies. The ability to differentiate human iPSCs into any cell type supports the study of biology and disease in these specified cells in vitro.

There is more to Biopreservation than storage on ice or freezing at a rate of -1°C/min. To comprehend the rationale behind Biopreservation Best Practices, a basic understanding of cellular response to cold and freezing is essential. This presentation will identify the critical process parameters (CPPs) of Cold Chain, such as freezing and thawing rates, storage and post-thaw stability, and container type, among others, and will discuss the impact of these CPPs on critical quality attributes (CQAs) such as viability, yield, proliferation rate, and return to function.

In the past decade, the synthetic biology market has seen fledgling companies rise to IPO smashing power players, as the demand for high-quality, engineered biomolecules grows exponentially. The synthesis of DNA molecules and libraries has always been at the vanguard of synthetic biology endeavors but has seen its share of challenges that scientists have to navigate. Technological advancements and democratization of synthesis modalities have expanded access to an even wider range of life science investigators—presenting new challenges, but also a host of opportunities for new solutions.