Reaction Biology Corporation
Reaction Biology Corp. (“RBC”) is a premier service provider for drug profiling, screening and early-stage drug discovery collaborations. Its proprietary HotSpot technology is an innovative platform for screening/profiling small molecules vs biological target assayable by radioisotope detection. For kinase and transferase enzymes in particular, such assays are the “gold standard”. RBC screens and profiles vs all major classes of epigenetic transferases via the radioisotope approach—HMTs, DNMTs, HATs, and also over 600 kinases. HotSpot assay substrates require no special modifications (e.g. biotin, fluorophors or other tags). Hence biologically relevant substrates may be used, including full nucleosomes, histone proteins or peptides. Since detection does not rely on antibodies, coupling enzymes or fluorescence, major causes of false positives, false negatives and compound interference are eliminated.