Home > Resources > Whitepapers > Is A Rapid Cooling Step Needed When Freezing Cells For Cryopreservation?

Is A Rapid Cooling Step Needed When Freezing Cells For Cryopreservation?

March 14, 2018

Successful cell cryopreservation requires a controlled cooling rate. Cooling too rapidly or too slowly will lead to a poorer outcome. This is true because of the physical environment cells experience during cryopreservation. As ice forms in the extracellular solution, pure water is locked away like ice. As a result, cells are suspended in an increasingly concentrated solution, which dehydrates them. Cooling too slowly makes this solution toxic; cooling too rapidly prevents cells from dehydrating sufficiently, and intracellular ice forms.

Spotlight

IDEXX BioAnalytics

IDEXX BioAnalytics provides research professionals in the biomedical, veterinary and life science fields comprehensive animal health monitoring, pathology services and veterinary clinical trial support, as well as microbiome and biological materials testing. With a global presence and a team of world-renowned scientists, clients can expect more. Quality that exceeds expectations, innovation that expands possibilities, and global support from IDEXX Labs—the industry leader in animal health care diagnostics. Partner with our world-class team of veterinarians, microbiologists, molecular biologists, and geneticists to streamline your research, generate reliable and reproducible data so you can test with confidence.