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ISOLATION AND EXPANSION OF MESENCHYMAL STROMAL OR STEM CELLS (MSCS) FOR TRANSLATIONAL AND CLINICAL RESEARCH

May 16, 2019 | (12:00PM)
USA (United States of America)
Human mesenchymal stem cells (MSCs) hold great promise for clinical use and cell therapy applications. To ensure highest quality and safety of the resulting cellular products, MSCs have to be maintained using standardized cultivation conditions and procedures. To this end, we have developed our xeno-free MSC-Brew GMP Medium following the recommendations of USP <1043> on ancillary materials, thus enabling isolation and expansion of MSCs from various tissue sources (e.g. human bone marrow, adipose tissue, and umbilical cord). To increase the level of process standardization and product safety we developed a procedure using the integrated cell processing platform CliniMACS Prodigy® for i) density gradient centrifugation (DGC) of human bone marrow, ii) cultivation of MSCs starting from bone marrow mononuclear cells (BM-MNCs), and iii) passaging of MSCs.