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QUANTIFY INTACT VIRUS PARTICLES MORE PRECISELY AND FASTER THAN QPCR

SHARESHARESHARE
An analytical quality by design (AQbD) approach was embraced for the development of a capillary zone electrophoresis (CZE) method for the quantification of intact virus particles. With CE, the intact adenovirus particles were separated from sample matrix components such as cell debris, residual cell DNA, proteins, and salts. The intact adenovirus particle concentrations obtained by CE were overall equivalent to those obtained by quantitative polymerase chain reaction (qPCR), although the CE method showed better precision (RSD < 6%) than anion-exchange HPLC and qPCR (10 – 25% RSD).