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IDENTIFY AND QUANTIFY INDIVIDUAL HOST CELL PROTEINS

SHARESHARESHARE
ELISA is the golden standard for HCP analysis but has some drawbacks. It only provides a single number (ng HCP/mg drug substance) and no information about the specific HCP’s. Here we present an HCP analysis method based on a SWATH LC-MS that provides reproducible identification and absolute quantification of each individual HCP as well as the total HCP content. The method is generic for vaccines, mAbs, small therapeutic proteins and protein biopharmaceuticals expressed in different cell lines and expression systems.