Sci-fi to reality: Superpowered salamander may hold the key to human regeneration

Phys.org | January 24, 2019

Regeneration is one of the most enticing areas of biological research. How are some animals able to regrow body parts? Is it possible that humans could do the same? If scientists could unlock the secrets that confer those animals with this remarkable ability, the knowledge could have profound significance in clinical practice down the road. Scientists at the University of Kentucky have taken this fantasy one step closer to reality, announcing today that they have assembled the genome of the axolotl, a salamander whose only native habitat is a lake near Mexico City. Axolotls have long been prized as models for regeneration, said Randal Voss, a professor in the UK Spinal Cord and Brain Injury Research Center and a co-PI on the project.
"It's hard to find a body part they can't regenerate: the limbs, the tail, the spinal cord, the eye, and in some species, the lens, even half of their brain has been shown to regenerate," he said. Though humans share many of the same genes with axolotl, the salamander genome is ten times larger, posing a formidable barrier to genetic analyses.

Spotlight

Peripheral blood mononuclear cells (PBMCs) are a heterogeneous the population of blood cells having round nuclei. They consist of monocytes, macrophages, dendritic cells and lymphocytes (T cells, B cells, and NK cells). In order to induce and upregulate the expression of specific immune checkpoint markers in the PBMC populations, we used Dynabeads®, polystyrene-coated microspheres with antibodies against human CD3 and CD28 proteins covalently coupled to the surface.

Spotlight

Peripheral blood mononuclear cells (PBMCs) are a heterogeneous the population of blood cells having round nuclei. They consist of monocytes, macrophages, dendritic cells and lymphocytes (T cells, B cells, and NK cells). In order to induce and upregulate the expression of specific immune checkpoint markers in the PBMC populations, we used Dynabeads®, polystyrene-coated microspheres with antibodies against human CD3 and CD28 proteins covalently coupled to the surface.

Related News

MEDTECH

Precision Coating Announces Merger with N2 Biomedical to Expand Medical Coatings Platform

N2 Biomedical | September 20, 2021

Precision Coating, a leading medical coatings service provider to interventional, orthopedic, and advanced surgical markets, is pleased to announce a merger with N2 Biomedical, a Bedford, MA-based company. N2 Biomedical, an Ampersand Capital Partners portfolio company, provides nano-engineered surface treatment and coating services to the medical device industry with its low-temperature Ion Implantation Surface Treatments and Ion Beam Assisted Deposition (IBAD) technologies. We are very excited to add N2's technologies and people to Precision Coating. N2 extends our coating platform technologies in ways that enhance our value proposition for customers. This is particularly true in orthopedics, where we can now offer engineered coating solutions across both instruments and implants. The two companies have a great deal of complementarity between market focus, customer overlap, product functional properties, and operational execution, not to mention geographic proximity. - Bill Ellerkamp, President of Precision Coating About Precision Coating For over 50 years, Precision Coating has collaborated with medtech partners to realize their product design and manufacturing goals by providing highly engineered coating and specialized finishing services. Among the portfolio of medical coatings and application solutions, GlideLine™ and InfiNiTiCoat™ coatings comprise the broadest offering of applied fluoropolymer (PTFE) coatings for interventional and advanced surgical devices, and the patented MICRALOX® portfolio is the recognized standard for superior aluminum anodizing finishes for barrier properties, corrosion resistance, and extended product life cycles, targeting orthopedic and advanced surgical instruments. Precision Coating partners with early-stage device innovators through major medical equipment manufacturers, deploying comprehensive solutions—from concept to commercialization—and offering flexible prototype work through high-volume coating application, finishing, and printing services. The company operates facilities in Massachusetts, Rhode Island, and Costa Rica, and serves customers worldwide, offering seamless supply chain support. About N2 Biomedical Established in 2013, N2 provides coating and surface treatment development and application services for implantable and other medical devices utilized in orthopedic, cardiovascular, and other healthcare end-markets. N2 is ISO 13485 certified, FDA GMP compliant, and operates in a 27,000-square-foot facility with laboratory, manufacturing, and cleanroom space to service all customer and regulatory requirements. The company leverages its proprietary processes and equipment to provide customized solutions that enhance the characteristics of various materials in critical applications, including lubricity, infection resistance, biocompatibility and tissue integration, and wear and corrosion resistance. About Ampersand Capital Partners Founded in 1988, Ampersand is a middle-market private equity firm with more than $2 billion of assets under management dedicated to growth-oriented investments in the healthcare sector. With offices in Boston and Amsterdam, Ampersand leverages its unique blend of private equity and operating experience to build value and drive superior long-term performance alongside its portfolio company management teams. Ampersand has helped build numerous market-leading companies across each of the firm's core healthcare sectors.

Read More

MEDICAL

GT Biopharma Announces FDA Data - GTB-3550, for Treatment of High-risk Myelodysplastic Syndromes

GT Biopharma | December 23, 2020

GT Biopharma, Inc. (OTCQB: GTBP) (GTBP.PA) an immuno-oncology organization focused in on innovative treatments dependent on the Company's restrictive NK cell engager (TriKE™) innovation stage is satisfied to report the introduction of extra interval information results for the Company's lead therapeutic competitor, GTB-3550, for the treatment of high-risk myelodysplastic syndromes (HR-MDS). Erica Warlick, M.D, Principal Investigator for the GTB-3550 clinical trial, introduced extra clinical data results with the treatment with HR-MDS persistent #7 of its TriKE™ GTB-3550 during the Q&A meeting following her introduction at the 62nd American Society of Hematology (ASH) Annual Meeting and Exposition. Mr. Anthony Cataldo, the Chairman and Chief Executive Officer of GT Biopharma commented, "Our clinical data demonstrates that our proprietary TriKE™ (CD16/IL15/CD33), safely activated and harnessed the patient's native NK cell's cancer killing ability in a target-directed fashion without side effects. Which is not the case with highly expensive and intrusive supplemental NK cell therapies. We look forward to progressing to the next level."

Read More

MEDICAL

Meridian Bioscience Launches Lyo-Ready™ qPCR Inhibitor Tolerant Blood-Specific Master Mixes for DNA & RNA Direct Detection

Meridian Bioscience, Inc. | March 31, 2022

Meridian Bioscience, Inc. (NASDAQ: VIVO), a leading global provider of diagnostic testing solutions and life science raw materials, announced today the launch of two new master mixes that detect DNA and RNA in crude blood samples down to a single copy – enabling the earliest detection of cancer using non-invasive liquid biopsy. These two innovative mixes, Lyo-Ready™ Direct DNA qPCR Blood Mix and Lyo-Ready™ Direct RNA/DNA qPCR Blood Mix, are designed for applications that require ultra-sensitive detection, such as liquid biopsy. This breakthrough technology is the first blood-specific, inhibitor-tolerant, and lyophilizable chemistry that enables extraction-free qPCR/RT-qPCR for liquid biopsy analysis at a performance level on par with traditional qPCR from purified nucleic acids. Liquid biopsy is a non-invasive alternative to tissue biopsy and offers ultra-sensitive detection of tumor-associated DNA or RNA from biofluids such as blood. Sample types like whole blood, serum, plasma, and the anticoagulants used for specimen stabilization contain PCR inhibitors that can significantly reduce detection sensitivity and may cause false-negative results. Inhibitors are usually removed by DNA/RNA extraction; however, that approach is very inefficient and increases assay time, cost, and the risk of contamination or error. Meridian's innovative Lyo-Ready™ Blood-Specific Master Mixes do not require extraction and are proven to excel with crude blood and its derivatives without the need to remove inhibitors. These groundbreaking mixes can help improve patient outcomes by enabling early cancer detection by delivering assay sensitivity down to one copy of DNA. Meridian's new mixes are formulated with lyophilization excipients, making them compatible with lyophilization to create room-temperature stable molecular diagnostic assays. Not only does this allow maximum sample input in the liquid biopsy analysis, but it also eliminates the need for cold chain storage of the finished test kit, limiting our customer's environmental impact. We are excited to expand our sample-specific molecular range with the market's first lyophilizable, and inhibitor-tolerant master mixes designed for direct amplification of nucleic acids from liquid biopsy. It is a truly disruptive technology that makes the early detection of cancer biomarkers directly from liquid biopsy a reality. We are proud of being the technology innovator enabling efficient and more cost-effective diagnostic assay development to help clinicians deliver the quickest, most accurate diagnosis for patients." Florent Chang-Pi-Hin, Ph.D., Vice President, Global Research and Development. About Meridian Bioscience, Inc. Meridian is a fully integrated life science company that develops, manufactures, markets, and distributes a broad range of innovative diagnostic products. We are dedicated to developing and delivering better solutions that give answers with speed, accuracy, and simplicity that are redefining the possibilities of life from discovery to diagnosis. Through discovery and development, we provide critical life science raw materials used in immunological and molecular tests for human, animal, plant, and environmental applications. Through diagnosis, we provide diagnostic solutions in areas including gastrointestinal and upper respiratory infections and blood lead level testing. We build relationships and provide solutions to hospitals, reference laboratories, research centers, veterinary testing centers, physician offices, diagnostics manufacturers, and biotech companies in more than 70 countries worldwide.

Read More