Engineered DNA vaccine protects against emerging Mayaro virus

Medical Xpress | February 07, 2019

Infections by the mosquito-borne Mayaro virus (MAYV) have largely been restricted to people who ventured into the heavily forested areas of Trinidad and Tobago and neighboring regions of South America. However, recent laboratory studies showing additional mosquito vectors can transmit MAYV as well as a reported case of MAYV infection in Haiti in 2015, reveal the potential for the virus to expand its range further into tropical regions of the Caribbean and Central and South America. Now, researchers reporting in PLOS Neglected Tropical Diseases have developed a novel vaccine capable of inducing protective immunity against MAYV. MAYV, which causes fever, rash, headache, nausea, and vomiting, was first identified in Trinidad in 1954. Some people who contract MAYV develop recurrent joint pain that can last for months after the initial infection. Over the years since its discovery, only sporadic cases have been reported, mostly in tropical areas of South America, but recent surveys have suggested it may be circulating in Central America and a case was confirmed in Haiti in 2015. MAYV infection causes fever, rash, headache, nausea, and vomiting In the new study, Kar Muthumani, director of the Laboratory of Emerging Infectious Diseases at The Wistar Institute, and colleagues used synthetic DNA technologies to design a DNA vaccine that encodes the sequence for the MAYV envelope (E) glycoproteins. The researchers optimized a consensus sequence representative of multiple MAYV strains (scMAYV-E) to account for the natural genetic variability in the main antigen on the surface of the virus.

Spotlight

This technical note demonstrates the comprehensive
characterization and confident identification of glucuronide
metabolites from hepatocyte incubations of midazolam. An
orthogonal fragmentation mechanism was applied to generate diagnostic fragment ions for confident identification of glucuronide metabolites

Spotlight

This technical note demonstrates the comprehensive
characterization and confident identification of glucuronide
metabolites from hepatocyte incubations of midazolam. An
orthogonal fragmentation mechanism was applied to generate diagnostic fragment ions for confident identification of glucuronide metabolites

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